polymerized lms isoforms 511 Search Results


polymerized lms isoforms 511  (BioLamina)
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BioLamina polymerized lms isoforms 511
<t>Polymerized</t> laminins <t>(polyLM)</t> 111, 411, and 511 trigger NET release. Neutrophils were incubated on polyLM 111, 411, and 511 (50 μg/mL)-coated plates for 90 min and then fixed and stained for histone ( A , F , K ); polyLM 111, 411, and 511 ( B , G , L ); and DNA ( C , H , M ). Enlarged images show merged staining for polyLM 111 ( D ), 411 ( I ), and 511 ( N ). Bars: 20 µm. NETs in the culture supernatants of neutrophils stimulated with polyLM 111 ( E ), 411 ( J ), and 511 ( O ) for 90 min were quantified using PicoGreen. The data were normalized according to spontaneous release of DNA (Nil) and are represented as the mean ± SEM of 4 experiments ( E , J , O ). * p < 0.05.
Polymerized Lms Isoforms 511, supplied by BioLamina, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Polymerized laminins (polyLM) 111, 411, and 511 trigger NET release. Neutrophils were incubated on polyLM 111, 411, and 511 (50 μg/mL)-coated plates for 90 min and then fixed and stained for histone ( A , F , K ); polyLM 111, 411, and 511 ( B , G , L ); and DNA ( C , H , M ). Enlarged images show merged staining for polyLM 111 ( D ), 411 ( I ), and 511 ( N ). Bars: 20 µm. NETs in the culture supernatants of neutrophils stimulated with polyLM 111 ( E ), 411 ( J ), and 511 ( O ) for 90 min were quantified using PicoGreen. The data were normalized according to spontaneous release of DNA (Nil) and are represented as the mean ± SEM of 4 experiments ( E , J , O ). * p < 0.05.

Journal: Biomedicines

Article Title: Laminin Triggers Neutrophil Extracellular Traps (NETs) and Modulates NET Release Induced by Leishmania amazonensis

doi: 10.3390/biomedicines10030521

Figure Lengend Snippet: Polymerized laminins (polyLM) 111, 411, and 511 trigger NET release. Neutrophils were incubated on polyLM 111, 411, and 511 (50 μg/mL)-coated plates for 90 min and then fixed and stained for histone ( A , F , K ); polyLM 111, 411, and 511 ( B , G , L ); and DNA ( C , H , M ). Enlarged images show merged staining for polyLM 111 ( D ), 411 ( I ), and 511 ( N ). Bars: 20 µm. NETs in the culture supernatants of neutrophils stimulated with polyLM 111 ( E ), 411 ( J ), and 511 ( O ) for 90 min were quantified using PicoGreen. The data were normalized according to spontaneous release of DNA (Nil) and are represented as the mean ± SEM of 4 experiments ( E , J , O ). * p < 0.05.

Article Snippet: To produce polymerized LMs (polyLM), isoforms 111 (Thermo Fisher Scientific, Waltham, MA, USA), 411 and 511 (BioLamina, Sundbyberg, Sweden) were diluted to 50 μg/mL in 20 mM sodium acetate (pH 4) containing 1 mM CaCl 2 .

Techniques: Incubation, Staining